The expression of Ra and Rc genes in these mutants are shown in
Fig. 2. The tested wild types SA418, SA419 and all the mutants
exhibited a PCR product of about 858 bp; the 2-bp (GT) deletion
generated a BamHI restriction site, resulting in two digested
products of 653 and 203 bp. This pattern was detectable in all the
purple-pericarp mutants derived from SA419, while no such
a pattern was obtained from white-pericarp SA418 and SA419, and
red-pericarp mutants derived from SA418 (Fig. 2A). Similar findings
were also reported by Wang and Shu (2007). These distinct PCR
products patterns confirm the expression of the Ra gene in purplepericarp
characteristic of NaN3-induced rice mutants.
The Rc gene is a domestication-related gene required for red
pericarp in rice (Furukawa et al., 2006). In the present study, all the
red-pericarp mutants derived from SA418 (exhibited recessive rc
allele of 153 bp) exhibited the dominant Rc allele (167 bp) (Fig. 2B).
As a result, only SA418 and SA419 showed white pericarp, while the
mutants M-66, M-71, M-80, M-69 and M-74 had red pericarp (Fig.1).
The expression of Ra and Rc genes in these mutants are shown inFig. 2. The tested wild types SA418, SA419 and all the mutantsexhibited a PCR product of about 858 bp; the 2-bp (GT) deletiongenerated a BamHI restriction site, resulting in two digestedproducts of 653 and 203 bp. This pattern was detectable in all thepurple-pericarp mutants derived from SA419, while no sucha pattern was obtained from white-pericarp SA418 and SA419, andred-pericarp mutants derived from SA418 (Fig. 2A). Similar findingswere also reported by Wang and Shu (2007). These distinct PCRproducts patterns confirm the expression of the Ra gene in purplepericarpcharacteristic of NaN3-induced rice mutants.The Rc gene is a domestication-related gene required for redpericarp in rice (Furukawa et al., 2006). In the present study, all thered-pericarp mutants derived from SA418 (exhibited recessive rcallele of 153 bp) exhibited the dominant Rc allele (167 bp) (Fig. 2B).As a result, only SA418 and SA419 showed white pericarp, while themutants M-66, M-71, M-80, M-69 and M-74 had red pericarp (Fig.1).
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