In thelaboratory,soilsamplesweredri

In thelaboratory,soilsamplesweredriedandsievedthrough100meshedsteel
mesh.Extractionofpesticidesfromsoilwasdoneaccordingtothemethoddescribed
by Singhetal.(1999). Airdriedsoilsample(50g)wastakenin100mlsolventof
hexane:ethylacetate(9:1v/v)inaconicalflask,pluggedwithcottonwoolandthen
keptonarotaryshakerat200–250rpmfor12h.Themixturewasfilteredandthe
remainswerereextractedtwicewithadditional50mlofthesamesolventmixture.
Finalextractswerepooledandcleanupofsampleswasdoneusingaglasscolumn
packedwithfivepercentdeactivatedaluminaandanhydroussodiumsulfate.The
columnwaselutedwithn-hexane:benzene(1:1,v/v).Aftercleanup,thefinalextracts
wereconcentratedtoneardrynessusingrotaryvacuumevaporator.
Quantitative analysisoftheOC-DDTsandOC-HCHswereundertakenusingan
Agilent model6890GasChromatograph(GC)equippedwithElectronCapture
Detector (Ni63). ThecolumnusedfortheanalysiswasHP-5capillarycolumnof
0.32 mmid,0.25 mm filmthicknessand35mlength.Theoventemperature
started from150 1C withholdtimefor1min,thenincreasedto200 1C atarateof
5 1C min1 with holdtimefor5minandfinallyto290 1C atarateof10 1C min1.
The injectionporttemperaturewaskeptat285 1C andthedetectortemperature
was at300 1C. Nitrogenwasusedascarriergasataconstantflowof1mlmin1.
Nitrogen wasalsousedasamakeupgasat75mlmin1. 1 ml volumeofsamples
was injectedinsplit/splitlessmode.
The linearrangeofthedetectorwasdeterminedfrominjectionofstandard
mixtures. CalibrationlineswereperformedforalltheOCPsandtheresulting
correlation coefficients(r2) forthecalibrationcurveswereallgreaterthan0.99.
The concentrationsofindividualOCswerethenquantifiedbycomparingthepeak
area oftheparticularcompoundinsampleextractstothatofthecorresponding
external standardafterreplicateanalysis.

ผลลัพธ์ (ไทย) 1:

ใน thelaboratory, soilsamplesweredriedandsievedthrough100meshedsteel
ตาข่ายExtractionofpesticidesfromsoilwasdoneaccordingtothemethoddescribed
โดย Singhetal(1999) . Airdriedsoilsample(50g) wastakenin100mlsolventof
inaconicalflask โพลี: ethylacetate(9:1v/v), pluggedwithcottonwoolandthen
keptonarotaryshakerat200–250rpmfor12hThemixturewasfilteredandthe
remainswerereextractedtwicewithadditional50mlofthesamesolventmixture.
Finalextractswerepooledandcleanupofsampleswasdoneusingaglasscolumn
packedwithfivepercentdeactivatedaluminaandanhydroussodiumsulfateThe
columnwaselutedwithn-hexane:benzene(1:1,v/v)Aftercleanup, thefinalextracts
wereconcentratedtoneardrynessusingrotaryvacuumevaporator.
เชิงปริมาณ analysisoftheOC-DDTsandOC-HCHswereundertakenusingan
Agilent model6890GasChromatograph equippedwithElectronCapture (GC)
(Ni63) เครื่องตรวจจับ ThecolumnusedfortheanalysiswasHP 5capillarycolumnof
mmid $ 0.32, filmthicknessand35mlength 0.25 mmTheoventemperature
เริ่มต้น from150 1C withholdtimefor1min, thenincreasedto200 1C atarateof
5 1 C ต่ำสุด 1 กับ holdtimefor5minandfinallyto290 1C atarateof10 C 1 นาที 1.
andthedetectortemperature 1C injectionporttemperaturewaskeptat285
ถูก at300 ซี 1 Nitrogenwasusedascarriergasataconstantflowof1mlmin 1.
wasalsousedasamakeupgasat75mlmin ไนโตรเจน 1 1 ml volumeofsamples
ถูก injectedinsplit / splitlessmode.
linearrangeofthedetectorwasdeterminedfrominjectionofstandard
ส่วนผสม CalibrationlineswereperformedforalltheOCPsandtheresulting
forthecalibrationcurveswereallgreaterthan0.99 coefficients(r2) สห.
concentrationsofindividualOCswerethenquantifiedbycomparingthepeak
oftheparticularcompoundinsampleextractstothatofthecorresponding ตั้ง
standardafterreplicateanalysis ภายนอก

ผลลัพธ์ (ไทย) 2:

ใน Singhetal. (1999) analysisoftheOC-DDTsandOC-HCHswereundertakenusingan Agilent model6890GasChromatograph (GC) equippedwithElectronCapture ตรวจจับ (Ni63) ThecolumnusedfortheanalysiswasHP-5capillarycolumnof mmid 0.32, 0.25 มม. filmthicknessand35mlength.Theoventemperature เริ่ม from150 1C withholdtimefor1min, thenincreasedto200 1C atarateof 1C 5 นาที? 1 ด้วย holdtimefor5minandfinallyto290 1C atarateof10 1C นาที? 1 injectionporttemperaturewaskeptat285 1C andthedetectortemperature เป็น 1C AT300 Nitrogenwasusedascarriergasataconstantflowof1mlmin? 1 ไนโตรเจน wasalsousedasamakeupgasat75mlmin? 1 volumeofsamples 1 มล. ถูก injectedinsplit / splitlessmode linearrangeofthedetectorwasdeterminedfrominjectionofstandard ผสม CalibrationlineswereperformedforalltheOCPsandtheresulting ค่าสัมประสิทธิ์สหสัมพันธ์ (r2) forthecalibrationcurveswereallgreaterthan0.99 concentrationsofindividualOCswerethenquantifiedbycomparingthepeak พื้นที่ oftheparticularcompoundinsampleextractstothatofthecorresponding standardafterreplicateanalysis ภายนอก

ผลลัพธ์ (ไทย) 3:

ใน thelaboratory soilsamplesweredriedandsievedthrough100meshedsteel
, ตาข่าย extractionofpesticidesfromsoilwasdoneaccordingtothemethoddescribed
โดย singhetal . ( 1999 ) airdriedsoilsample ( 50 กรัม ) wastakenin100mlsolventof
เฮกเซน : เอธิลอะซีเตต ( 9:1v / V ) inaconicalflask pluggedwithcottonwoolandthen , keptonarotaryshakerat200 – 250rpmfor12h themixturewasfilteredandthe

.remainswerereextractedtwicewithadditional50mlofthesamesolventmixture .
finalextractswerepooledandcleanupofsampleswasdoneusingaglasscolumn packedwithfivepercentdeactivatedaluminaandanhydroussodiumsulfate
.
columnwaselutedwithn เฮกเซน : เบนซิน ( 1 : 1 v / v ) aftercleanup thefinalextracts

, wereconcentratedtoneardrynessusingrotaryvacuumevaporator .ปริมาณ analysisoftheoc ddtsandoc hchswereundertakenusingan
Agilent model6890gaschromatograph ( GC ) เครื่องตรวจจับ equippedwithelectroncapture
( ni63 ) thecolumnusedfortheanalysiswashp-5capillarycolumnof
0.32 mmid filmthicknessand35mlength , 0.25 มิลลิเมตร theoventemperature
เริ่ม from150 withholdtimefor1min 1B , 1C atarateof
thenincreasedto2005 ในมิน  1 กับ holdtimefor5minandfinallyto290 1C atarateof10 1C มิน  1

injectionporttemperaturewaskeptat285 1C andthedetectortemperature เป็น at300 1c nitrogenwasusedascarriergasataconstantflowof1mlmin  1 .
ไนโตรเจน wasalsousedasamakeupgasat75mlmin  1 1 ml volumeofsamples
คือ injectedinsplit / splitlessmode .

linearrangeofthedetectorwasdeterminedfrominjectionofstandard ผสมcalibrationlineswereperformedforalltheocpsandtheresulting
ค่าสัมประสิทธิ์สหสัมพันธ์ ( R ) forthecalibrationcurveswereallgreaterthan0.99 .


standardafterreplicateanalysis oftheparticularcompoundinsampleextractstothatofthecorresponding concentrationsofindividualocswerethenquantifiedbycomparingthepeak พื้นที่ภายนอก