2.5. Experimental design
Response surface methodology (RSM) with central composite
design (CCD) was employed to optimise the extraction parameters,
viz. concentration of ethanol (X1), incubation temperature (X2) and
solvent-to-solid ratio (X3). The range and central point value of all
the three process variables are shown in Table 1.
The process variables were coded according to the following
equation
x 1⁄4 ðX i À X 0 Þ=DX
where x is the dimensionless coded value, Xi is the actual value of
variables, X0 is the actual value of variables at the centre point,
and DX is the step change value. After the conduct of the experi-
ment, the data were fitted with a second-order polynomial equation
as follow
2.5. Experimental designResponse surface methodology (RSM) with central compositedesign (CCD) was employed to optimise the extraction parameters,viz. concentration of ethanol (X1), incubation temperature (X2) andsolvent-to-solid ratio (X3). The range and central point value of allthe three process variables are shown in Table 1.The process variables were coded according to the followingequationx 1⁄4 ðX i À X 0 Þ=DXwhere x is the dimensionless coded value, Xi is the actual value ofvariables, X0 is the actual value of variables at the centre point,and DX is the step change value. After the conduct of the experi-ment, the data were fitted with a second-order polynomial equationas follow
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