Although circulating exosomes and their content can serve as biomarkers
for various diseases, the standard ultracentrifugation based
exosome isolation methods established for research purposes are
time-consuming and labour intensive and may not be suitable for use
in clinical laboratories. Recently, alternative methods for exosomeisolation
with reduced hands-on time and without the requirement for
specialised expensive equipment have been developed. For instance,
precipitation based methods have been used for biomarker discovery
studies [7] and a microfiltration based method for the isolation of urinary
exosomes has been introduced [8].