The Supelcosil LC-18-DB HPLC reversed-phase column
(3lm particle size and 250·3.9 ID) was utilized for the
detection of vitamin C and MDA levels. While mobile
phase (3.7 mM phosphate buffer, pH 4.0) was used
1.0 ml min
1
flow rate to vitamin C level, the free MDA
level was determined using mobile phase consisting of
30 mM KH2PO4as a buffer, H3PO4
with pH 4 and methanol (65–35% v/v) at 1.5 ml min
1
flow rate. Each run was
repeated three times to check repeatability