The carboxylate anion of Asp32 increases the proton affinity of the imidazole ring of His64 to an extent. Meanwhile, His64 captures the proton from the hydroxyl group of Ser221 and Ser221 finally attacks the peptide bond.
The carboxylateanion of Asp32 increases the proton affinity of the imidazole ring ofHis64 to an extent. Meanwhile, His64 captures the proton from thehydroxyl group of Ser221 and Ser221 finally attacks the peptide bond.