Yeast isolated from fruits were than fermented by optimizing the medium (MGYP) by varying the glucose concentration (5, 10, 30, 50, 70g/l) in an incubating shaker at 30oC in 250mL flasks, after 72 h. The yeast isolated from apple showed maximum yeast biomass (0.38g/100mL) at 70g/L glucose concentration and minimum on 50g/l (0.03g/100mL) glucose concentration.