Agar disks (6-mm diameter) from actively growing cul-
tures of
P. infestans
were plated on juice-V8 agar media
(90
9
15 mm Petri dishes), with and without Xcn1. Petri
dishes were incubated at 18
±
1
°
C in darkness. Colony
diameter was measured after 2 weeks incubation. All
sporangia were removed from the agar medium, and spo-
rangia on each Petri dish were counted via microscopic
enumeration. Four replicates for each Xcn1 concentration
were used, and the experiment was repeated three times