Lipid hydroperoxides were determined according to the International
Dairy Federation method slightly modified to suit meat analysis (Shantha
& Decker, 1994). Lipids (0.01–0.30 g depending on the extent of peroxidation)
extracted from meat as described above (Lee et al., 1996), were
mixed with 9.8 ml chloroform-methanol in a disposable glass tube and
vortex-mixed briefly. Ammonium thiocyanate solution (0.05 ml) was
added and the sample was again vortex-mixed briefly. Then 0.05 ml
iron (II) solution was added, the sample was again vortex-mixed,
and after 5 min incubation at room temperature, the absorbance
was measured at 500 nm. The results were expressed in milliequivalents
of peroxides/kg meat.
Malondialdehyde (MDA) was determined by a selective third-order
derivative spectrophotometric method previously described (Botsoglou
et al., 2012). MDA concentration was expressed in ng/g meat.
Lipid hydroperoxides were determined according to the International
Dairy Federation method slightly modified to suit meat analysis (Shantha
& Decker, 1994). Lipids (0.01–0.30 g depending on the extent of peroxidation)
extracted from meat as described above (Lee et al., 1996), were
mixed with 9.8 ml chloroform-methanol in a disposable glass tube and
vortex-mixed briefly. Ammonium thiocyanate solution (0.05 ml) was
added and the sample was again vortex-mixed briefly. Then 0.05 ml
iron (II) solution was added, the sample was again vortex-mixed,
and after 5 min incubation at room temperature, the absorbance
was measured at 500 nm. The results were expressed in milliequivalents
of peroxides/kg meat.
Malondialdehyde (MDA) was determined by a selective third-order
derivative spectrophotometric method previously described (Botsoglou
et al., 2012). MDA concentration was expressed in ng/g meat.
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