Nanocrystalline copper oxides (CuO) were immobilized onto the woven and non-woven fabrics at room temperature by reactive DC magnetron sputtering. XRD pattern revealed the immobilization of CuO onto the fabrics. The antimicrobial test for the resultant CuO-fabric composites was performed against both Staphylococcus aureus and Escherichia coli bacterium using Agar diffusion plate test. The killing efficiency of bacteria and conformations of bacterial cell damage was noticed