callus extracts revealed that the callus established in MS medium supplemented with 4.5 μM NAA and 0.46 μM BAP produced the highest yield of Proscillaridin A (4.51 mg/g DW), Scilliroside (3.3 mg/g DW), Scillaren A (2.35 mg/g DW) and desacetylscilliroside (8.62 mg/g DW), which was higher than from the intact plants of Charybdis congesta[18]. Plant tissue culture systems can be used for continuous production of secondary metabolite compared to whole plants which shows more variation based on physiological and geographical conditions. The quantity of active principle produced can also be increased by changing the concentration of the inducer. Further this approach can considerably decrease the exploitation of medicinal plants where the entire plant is medicinal.