Due to the instability of sugarcane flowering and pollen viability in São Paulo State, techniques that artificially synchronise flowering and maintain pollen viability have a great impact on sugarcane breeding programmes. Moreover, the inductive photoperiod of the Brazilian sugarcane genotypes is still unknown mainly due to a lack of studies related to flowering induction at photoperiod facilities. Therefore, the present study aimed to study the behaviour of 16 sugarcane genotypes using different inductive flowering photoperiod treatments. The results will contribute to synchronise flowering to planning crosses and also help breeders in genetic introgression programmes between commercial sugarcane cultivars and wild sugarcane species, especially those from Saccharum spontaneum.