A more physiologically relevant approach to determining AC is
to use digestive enzymes (pepsin, pancreatin, alpha-amylase,
lipase, etc) instead of aqueous-organic extraction solvents to
determine AC in both the supernatant (AC3) from enzyme digestion
and the corresponding residues (AC4). Some AC values obtained by
this methodology are listed in Table 2. Note that, in this case, AC3 is
higher than AC4, which suggests that digestive enzymes trigger the
release of antioxidants from the food matrix more efficiently than
aqueous-organic solvents (AC3 > AC1) (Pe´ rez-Jime´nez and Saura-
Calixto, 2005; Serrano et al., 2007.