Zucchini seeds (Cucurbita pepo L. cv. Tempra) (Società Agricola Italiana Sementi—SAIS, Cesena, Italy) were germinated in a 2:1 (v:v) mixture of BRILL-Type 3 propagation substrate (Gebr. Brill Sub- strate GmbH & Co. KG, Georgsdorf, Germany) and sand on February
25, 2013.