Procedure (Female)
When handling and exposing the female, remember the hen is delicate and must be treated gently. Hold and stimulate her in much the same way as the male. As the operator applies pressure after the preliminary stroking and massage, the vent exerts and an orifice appears on the left side. It may be a round rosette or a cleft or skin overfold. This orifice, shown in Figure 4, is the oviduct’s terminal opening. An assistant should place the semen ¼ to 1 inch deep into this opening with a 1 cc syringe, a medicine dropper or similar device.
When making individual mating - one male with one female - use the entire semen collection. Various studies show, however, that good results can be achieved with as little as 0.05 cc of semen per insemination.
Relax pressure on the female’s body as soon as possible after insemination so the oviduct can return to its normal position, drawing the semen inward.