Thus in conclusion, the transformation vector and technologies presented here allow not only more efficient transformation of microalgae, but also the creation of self-cloned, safe transgenic algae by adding endogenous genes intended for overexpression 5′ and 3′ to the endogenous selection marker. Such transformation technologies for metabolic engineering of high value product accumulation, TAG or biomass productivity are essential tools for enhancing the impact of algal biotechnology in the future.