The concentrated homogenate was applied to a Diaion HP-20 (5 i.d. × 50 cm) column and eluted with
H2O, MeOH/H2O (40:60, v/v), MeOH, and acetone, successively, to give the corresponding H2O (116 g), MeOH/H2O (40:60) (52 g),MeOH (12.4 g), and acetone (0.629 g) fractions, respectively