In this study, all strains of lactobacilli and bifidobacteria were able to produce lactic acid at total concentrations ranging from 3.50 to 4.75 mg/mL. Beneficial effects of lactic acid on skin such as improving skin barrier function, hydration and lightening have been extensively reported. It is also reported that topical application of lactic acid at low concentrations of 0.1–1 % could exhibit antibacterial activity against most dermal pathogenic bacteria(Pasricha et al.1979). Our current study also showed that strains of lactobacilli and bifidobacteria were able to produce acetic acid at total concent rations ranging from 1.84 to 3.32 mg/mL. Nagoba et al. (2008) reported that topical application of acetic acid at a low concentration of 0.5 % successfully eliminated Pseudomonas aeruginosa from burns and soft tissue wounds of 14 out of 16 patients within 2 week