For comparison with the saccharification yield from lipid-extracted residual biomass, whole cells of Chlorella sp. KR-1
were subjected to saccharification using enzymatic and dilute acid methods (Table 3). A yield of 22.7% was obtained through enzymatic saccharification, while a yield of 93.3% was obtained using the dilute acid saccharification with an acid concentration of 0.3 N HCl. With regard to enzymatic saccharification, a high saccharification yield was obtained using residual biomass rather than the whole cells since the residual biomass had already been disrupted by DMC and methanol mixture during lipid extraction. The cell disruption after lipid extraction was confirmed by comparing the cell morphology of intact cell and solvent-treated cell based on scanning electron microscopic analysis (data not shown).