Non-catalyzed steaming process for samples 6x were conducted at 121 ± 3C in an autoclave with no final decompression took place. Samples were placed on wire-mesh container to allow thor-ough steaming on the samples. Since the steaming process is con-ducted in a closed reactor where interruption for withdrawing sample is not possible, three steaming cycles were carried out at three different retention time, namely 30, 60 and 90 min. 20 g of fresh water hyacinth sample was used in each cycle