Pulp and paper industry primary sludge being largely composed of lignocellulosic fibres, it
could be used as carbon source by bacteria having cellulolytic capability. The aim of this
study was to evaluate the use of cellulose contained in this type of sludge for Clostridium
thermocellum to produce ethanol, hydrogen and cellulases. In an ATCC 1191 medium containing
5 kg m3 dry primary sludge from recycled paper mill, batch culture reached stationary
phase after 2 days. All of the available cellulose was hydrolysed after 60 h of
incubation, with a final pH of 5.83. Metabolites produced after 60 h of fermentation were
acetate (8.50 mol m3), ethanol (11.30 mol m3), lactate (8.75 mol m3), formate
(0.27 mol m3), hydrogen (11.20 mol m3) and carbon dioxide (18.41 mol m3). Cellulase
activity was detected in the supernatant after 36 h, with a maximal activity of 0.25 U cm3
at 72 h. Pulp and paper primary sludge appeared to be a readily usable substrate for C.
thermocellum at this concentration, yielding both potential biofuels (hydrogen and ethanol)
as well as active cellulases.
Pulp and paper industry primary sludge being largely composed of lignocellulosic fibres, itcould be used as carbon source by bacteria having cellulolytic capability. The aim of thisstudy was to evaluate the use of cellulose contained in this type of sludge for Clostridiumthermocellum to produce ethanol, hydrogen and cellulases. In an ATCC 1191 medium containing5 kg m3 dry primary sludge from recycled paper mill, batch culture reached stationaryphase after 2 days. All of the available cellulose was hydrolysed after 60 h ofincubation, with a final pH of 5.83. Metabolites produced after 60 h of fermentation wereacetate (8.50 mol m3), ethanol (11.30 mol m3), lactate (8.75 mol m3), formate(0.27 mol m3), hydrogen (11.20 mol m3) and carbon dioxide (18.41 mol m3). Cellulaseactivity was detected in the supernatant after 36 h, with a maximal activity of 0.25 U cm3at 72 h. Pulp and paper primary sludge appeared to be a readily usable substrate for C.thermocellum at this concentration, yielding both potential biofuels (hydrogen and ethanol)as well as active cellulases.
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