To identify the siderophore, the bacterial culture was
extracted with one volume of dichloromethane by vigorous
shaking. After centrifugation at 3,0009g for 15 min, the
lower organic layer was evaporated. Thin layer chromatography
(TLC) was performed on aluminum-backed plates
of Silica Gel 60 F254 TLC plate (Merck 5554).