Most separations employ linear (edge-to-edge) with occasional use of radial (center-to-edge or edge-to-center) development. Two-dimensional development is the result of two consecutive linear developments in orthogonal directions with removal of the mobile phase between developments, and uses standard linear developing chambers [16]. In any of these modes, continuous or multiple development can be used to improve the separation of mixtures with a narrow polarity range (continuous development) or wide polarity range(multiple development) [6,17–19].