The main target of this research was to find out the ethanol presence in the solution that means the establishment of ethanol production procedure from spirulina. According to this it can be easily said that our result is positive. And this research is successful. Although our main objective was to set up a definite process for ethanol production from micro algae spirulina by using its carbohydrate content but we had a secondary objective of process standardization. We have made various samples to fulfill our secondary objective. Four types or sample variation were done by us.