Characterization of properties
Homogeneity of obtained fractions was tested using
analytical technique – thin layer chromatography (TLC). On
the glass slides coated with 0.25 mm layer of silica gel
Kieselge l60 (Merck) a drop of an aqueous solution of AA
and peptides were applied with a glass pipette, at a distance
of 10mm from the edge of the slide and from each
other. In order to develop the chromatograms, the follow -
ing systems were used: BuOH : acetic acid : water (BAW
4 : 1 : 5 v/v/v) and EtOAc : EtOH : NH3 (20 : 12 : 8 : 2 v/v/v/v).
Chromatograms were induced by iodine vapor and using
a solution of ninhydrin [16].