Before the experiment, nauplii of L. vannamei were raised in
a 20 m 3 (stocking density of 100 larvae L − 1 ), semi-cylindrical
hatchery tank in salinity of 35 ppm until they reached mysis1.
The microalgae Chaetoceros muelleri (5 ×10 4 cells mL − 1 ) was
added to the culture water daily. When the larvae reached the
stage of M1 (average dry weight of 0.085 ± 0.004 mg and average
length of 3.543 ± 0.076 mm) they were transferred to the experimental units, which were initially filled with water from the
hatchery tank. This line was free of any pathogens that require notification of the International Organization of Epizootics (from
Aquatec LTDA, Rio Grande do Norte, Canguaratema, Brazil).
Before the experiment, nauplii of L. vannamei were raised ina 20 m 3 (stocking density of 100 larvae L − 1 ), semi-cylindricalhatchery tank in salinity of 35 ppm until they reached mysis1.The microalgae Chaetoceros muelleri (5 ×10 4 cells mL − 1 ) wasadded to the culture water daily. When the larvae reached thestage of M1 (average dry weight of 0.085 ± 0.004 mg and averagelength of 3.543 ± 0.076 mm) they were transferred to the experimental units, which were initially filled with water from thehatchery tank. This line was free of any pathogens that require notification of the International Organization of Epizootics (fromAquatec LTDA, Rio Grande do Norte, Canguaratema, Brazil).
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