n order to research the relationship between endophytic fungus and active ingredients in medicinal
Paeonia suf fruticosa, a total of 57 fungal isolates were isolated from the roots, stems, leaves and buds of
medicinal plant Paeonia ostii ; mycelium wa s collected af ter these fungal isolates were fermented on PDA
medium for a few days; then the mycelium products were extracted; their extracts were analyzed by gas
chromatography – mass spectrometry. With this method, a strain endophytic fungi named J1-2 which can
produce paeonol wa s screened. Paeonol produced by J1-2 wa s analyzed by using a high resolution mass
spectrometer (HRMS) and nuclear magnetic resonance (NMR). The potential paeonol-procucing named
J1-2 wa s identi fied Chaetomium based on morphological characteristics and ITS sequence analysis. The
current research initially indicates that endophytic fungi can affect the potency of peony. At the same
time it also indicates that the numerous endophytic fungi inside the medicinal Paeonia suf fruticosa are
precious resource for the pharmaceutical natural products that are originally from the Paeonia
suf fruticosa.