In vitro clonal propagation of biodiesel plant
(Jatropha curcas L.)30
Datta et al. had reported the in vitro clonal
propagation of biodiesel plant (Jatropha curcas L.). In
this investigation, in vitro clonal propagation of sevenmonth-
old Jatropha curcas L. was achieved employing
nodal explants. Axillary shoot bud proliferation was
best initiated on Murashige and Skoog’s (MS)31 basal
medium supplemented with 22.2 μM N6-
benzyladenine (BA) and 55.6 μM adenine sulphate, in
which cultures produced 6.2 ± 0.56 shoots per nodal
explants with 2.0 ± 0.18 cm average length after 4–6
weeks. The rate of shoot multiplication was
significantly enhanced after transfer to MS basal
medium supplemented with 2.3 μM 6-furfuryl amino
purine (Kn), 0.5 μM indole- 3-butyric acid (IBA) and
27.8 μM adenine sulphate for 4 weeks. Both shoot
number (30.8 ± 5.48) and average shoot length (4.8 ±
0.43 cm) were found to increase significantly.32About
52% of root induction occurred in MS basal medium
supplemented with 1.0 μM IBA in 2–3 weeks. Further
elongation of roots with average length of 8.7 ± 1.35 cm
was obtained in unsupplemented MS basal medium for
2–3 weeks. The plantlets (12–16-week-old) were
successfully acclimatized in soil with 87% survival
frequency.