Sixty H. pylori strains were collected from the National
Cheng-Kung University Hospital, Tainan, Taiwan. H. pylori
J99, 26695, and ATCC43054 were purchased from ATCC. H.
pylori was grown on CDC anaerobic blood agar or Brucella
agar plates (Becton, Dickinson and Company) containing
10% horse serum (abbreviated as BAH) at 37C under
microaerophilic conditions. All bacteria were stored at
e80C in braineheart infusion (BHI) with 30% glycerol.