The experiment was carried out at the Department of Horticulture. Kasetsart University. Tissue culture plants of K.Khai were cultured on MS media supplementing with 4mg/l BA for each treatment. One hundred of K.Khai plantlets were soaked in 0.5.0.75, colchicines and 15 M. 30 M. 4 M of oryzaline containing DMSO for 2.5. 5.0 and 7.5 hours while the control was soaked in distilled water The explants were washed after soaked in distilled water and then cultured in modified MS medium with 4 mg/l BA. Subcultures of selected plants were prepared every month.