Bioremediation of textile dyestuffs under solidstate
fermentation (SSF) using industrial wastes as substrate
pose an economically feasible, promising, and eco-friendly
alternative. The purpose of this study was to adsorb Red M5B
dye, a sample of dyes mixture and a real textile effluent on
distillery industry waste-yeast biomass (DIW-YB) and its
further bioremediation using Bacillus cereus EBT1 under
SSF. Textile dyestuffs were allowed to adsorb on DIW-YB.
DIW-YB adsorbed dyestuffs were decolorized under SSF by
using B. cereus. Enzyme analysis was carried out to ensure
decolorization of Red M5B. Metabolites after dye degradation
were analyzed using UV–Vis spectroscopy, FTIR, HPLC, and
GC-MS. DIW-YB showed adsorption of Red M5B, dyes
mixture and a textile wastewater sample up to 87, 70, and
81 %, respectively. DIW-YB adsorbed Red M5B was decolorized
up to 98 % by B. cereus in 36 h. Whereas B. cereus
could effectively reduce American Dye Manufacture Institute
value from DIW-YB adsorbed mixture of textile dyes and
textile wastewater up to 70 and 100 %, respectively. Induction
of extracellular enzymes such as laccase and azoreductase
suggests their involvement in dye degradation. Repeated utilization
of DIW-YB showed consistent adsorption and ADMI
removal from textile wastewater up to seven cycles. HPLC
and FTIR analysis confirms the biodegradation of Red M5B.
GC-MS analysis revealed the formation of new metabolites.