Isolation and Enumeration of Bacteria
Isolation and enumeration of bacteria were performed by soil dilution plate technique using Bushnell Haas agar
media [9]. One gram of dried soil was dissolved in 9ml of distilled water and agitated vigorously. Different aqueous
dilutions 10-1, 10-2…10-10 of the suspension were applied onto plates and 20ml melted medium at around 50°C was
added to it. After gently rotating, the plates were incubated at 37°C for 24hrs. Enumeration of different isolates was
carried out selected colonies of bacteria were transferred from mixed culture plates onto respective agar plates and
incubated at 37°C for 24hrs plates containing pure cultures were stored at 4°C until the examination.