When presaccharification and fermentation processes were peformed at 37 C, the final ethanol concentration was 6.12 g/L for S.cerevisiae LBM-1 and 6.14 g/L for K. marxianus UFV-3 (Fig. 3A). At
42 C, the two strains exhibited the same fermentation behavior,and similar ethanol concentrations were obtained at the end of the
process (Fig. 3B). However, with presaccharification at 50 C followed by fermentations at 37 or 42 C, higher ethanol concentrations were attained for both species (Fig. 3C and DHigher ethanol yields were observed when fermentation was, indicating that for the two strains, the presaccharification
temperature is more important to ethanol yield than the fermenttion temperature. When analyzing the data showed in Fig. 2 and in
Table 2, it can be seen that ethanol yield depends on the initial glu
initiated after presaccharification at 50 C than at 37 or 42 C (cose concentration, as higher yields were achieved when fermentan was initiated with a higher glucose concentration. To confirm
this result, another SSF experiment was carried out in which fermentation was started after 72 h of presaccharification at 50 C
with an initial yeast concentration equal to an OD600nm of 2. Under
these conditions, the final ethanol concentration was approximately 15 g/L (Fig. 3E and F). Thus, it was clear that the ethanol
yield was higher when fermentation was started after 72 h of presaccharification compared to 24 h (Table 2) due to the higher
glucose concentration at the start of fermentation
When presaccharification and fermentation processes were peformed at 37 C, the final ethanol concentration was 6.12 g/L for S.cerevisiae LBM-1 and 6.14 g/L for K. marxianus UFV-3 (Fig. 3A). At
42 C, the two strains exhibited the same fermentation behavior,and similar ethanol concentrations were obtained at the end of the
process (Fig. 3B). However, with presaccharification at 50 C followed by fermentations at 37 or 42 C, higher ethanol concentrations were attained for both species (Fig. 3C and DHigher ethanol yields were observed when fermentation was, indicating that for the two strains, the presaccharification
temperature is more important to ethanol yield than the fermenttion temperature. When analyzing the data showed in Fig. 2 and in
Table 2, it can be seen that ethanol yield depends on the initial glu
initiated after presaccharification at 50 C than at 37 or 42 C (cose concentration, as higher yields were achieved when fermentan was initiated with a higher glucose concentration. To confirm
this result, another SSF experiment was carried out in which fermentation was started after 72 h of presaccharification at 50 C
with an initial yeast concentration equal to an OD600nm of 2. Under
these conditions, the final ethanol concentration was approximately 15 g/L (Fig. 3E and F). Thus, it was clear that the ethanol
yield was higher when fermentation was started after 72 h of presaccharification compared to 24 h (Table 2) due to the higher
glucose concentration at the start of fermentation
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