A serial concentrations (5 pmol, 4 pmol, 3 pmol, 2 pmol and 0 pmol) of p35S- GFP (Plasmid) or PCR-fragments of 35S-GFP-NOS (PCR-TES) were used to titrate out the transformation efficiency. Dots were blotted with anti-GFP antibody. The anti-actin shows the internal control.