For shoot induction, MS medium was supplemented with
different concentrations of BAP ranging from 1 to 10
mg/L. The best shoot induction response (90%) was
obtained in MS medium containing 10 mg/L BAP. At this
concentration, all the explants showed shoot induction
response within 8 days of inoculation from apical
meristem (Table 1; Figure 1b). With the increase in the
concentration of BAP, the time taken for shoot induction
was decreased while the rate of shoot induction, shoot
length and number of shoots per explants was increased.
The other medium used for shoot induction was BAP +
IAA. Best shoot induction response (80%) was obtained
in MS medium containing 8.8 µM BAP + 2.87 µM IAA
within 12 days of inoculation from vegetative buds.