a b s t r a c t
c-amino butyric acid (GABA) has antihypertensive and anti-stress effects on human health. In this study,
we developed a simple method for GABA production from sodium glutamate using barley bran without
any buffer or any coenzyme. We determined the optimal reaction conditions of the GABA production
using barley bran as described below, reaction temperature: 20 C, initial barley bran concentration:
150 mg/mL, initial sodium glutamate concentration: 10 mM, reaction time: 6 h. Under optimal conditions
using barley bran, the concentration of GABA reached 11.0 mM (containing GABA from barley bran) from
10.0 mMof sodium glutamate; however in using wheat and rice bran, the GABA concentrations were only
3.9 and 0.8 mM, respectively. To apply the water containing GABA produced from sodium glutamate
using barley bran (WGBB) to beer brewing, we prepared wort using WGBB. The wort contained a large
amount of GABA (257 lg/mL) without lowering the other wort qualities.
a b s t r a c tc-amino butyric acid (GABA) has antihypertensive and anti-stress effects on human health. In this study,we developed a simple method for GABA production from sodium glutamate using barley bran withoutany buffer or any coenzyme. We determined the optimal reaction conditions of the GABA productionusing barley bran as described below, reaction temperature: 20 C, initial barley bran concentration:150 mg/mL, initial sodium glutamate concentration: 10 mM, reaction time: 6 h. Under optimal conditionsusing barley bran, the concentration of GABA reached 11.0 mM (containing GABA from barley bran) from10.0 mMof sodium glutamate; however in using wheat and rice bran, the GABA concentrations were only3.9 and 0.8 mM, respectively. To apply the water containing GABA produced from sodium glutamateusing barley bran (WGBB) to beer brewing, we prepared wort using WGBB. The wort contained a largeamount of GABA (257 lg/mL) without lowering the other wort qualities.
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