3. B-complex vitamins and lysine
Thiamine was analysed using a Fluorometer (Toshniwal, Instruments, Pvt. Ltd., Ajmer, India) and niacin was analysed by a colorimetric method using a double beam spectrophotometer, 2203 (Systronics, Ambalal Sarabhai Enterprises Ltd., New Delhi, India) by following the standard methods of the AOAC (2000). Lysine content was estimated according to the method described by Miyahara and Jikoo (1967).