The developed multiplex PCR system is effective and timesaving for tracking GM soybeans. This screening method is based on 9 screening targets. These targets were successfully tested using all of the available CRMs of GM soybean, which contained the screening elements and 26 soybean-containing food products. The newly developed screening method will cover all potential GM events in soybean that can be present in a sample, as shown in the workflow. This strategy will help monitor and track authorized/unauthorized GM soybean in markets worldwide.