Various cellulosic substrates (cellobiose, carboxy-
methyl cellulose (CMC) and CM-cellulose) and glucose were
tested as a carbon source on cellulase production. The
maximum growth was exhibited in the medium containing
cellobiose, followed by glucose, CMC and least with CM-
cellulose. However, the highest cellulase was produced in the
medium containing CMC and the enzyme activity was 0.23
UmL’I at day 5 of cultivation whereas cellobiose and CM-
cellulose resulted in enzyme activity ofO. 15 and 0.09 UmL ',
respectively. However, the lowest enzyme production was
found in the medium containing glucose (0.05 UmL") (Figure
2). This result can be correlated with previous report that
mentioned about an increase in endoglucanases activity
when Trichndermu sp , was grown in the presence of CMC
(Aslam e! 111., 2010). Some previous studies also reported
that CMC is the substrate of choice for endoglucanase pro
duction (Shambe, 1987; Gautam, 2010). Moreover, it was
found that the cellulase production was repressed when C.
easanenszs JK»8 was grown in the medium containing
glucose, suggesting that cellulase production was under
catabolite»repression as has been reported for Alternaria
solani earlier by Sands and Lukens (1974), where a decreased
production of cellulases was observed in cellulose-deficient
medium.