The effects of electro-stimulation on heterotrophic denitrifying bacterial activity and nitrate removal
were investigated using a bench-scale bio-electrochemical reactor in this study. Results showed that
the maximum nitrate removal efficiency was 100% at the optimum current density of 200 mA/m2, at
which low nitrite production and high ATP aggregate level were obtained. The activity of denitrifying bacteria
was highest at the range densities of 200–250 mA/m2, although the terminative pH increased to
8.62 at 200 mA/m2 and 9.63 at 250 mA/m2. This demonstrates that suitable current densities could
improve the activity of denitrifying bacteria. Therefore, this study provides a number of useful information
to improve the bio-electrochemical reactor designs and promote the removal efficiency of pollutants.