Estimation of Cellulose, hemicelluloses and lignin
content:
Shoot cellulose content was estimated by taking
200 mg of dry powder with 3 ml of acetic-nitric
reagent (150 ml of 80% acetic acid with 15 ml of
concentrated nitric acid) and mixed in a vortex
(Thimmaiah, 1999). The tubes were placed in waterbath
for 30 min, cooled and then centrifuged for 15-
20 min. The supernatant was discarded. The residue
was washed with distilled water. Then 10 ml of 67 %
H2SO4 was added and allowed to stand for 1 h. The
above solution was diluted to 100 ml. One ml of
diluted solution was mixed with 4 ml of anthrone