where []MRE is the calculated mean residue ellipticity
(deg cm2 dmol−1); MRW, the mean residue weight for the
peptide bond [MRW is calculated as MRW = M/N-1,
where M is the molecular mass of the polypeptide chain
(Da), and N is the number of amino acids in the chain];
obs, the observed ellipticity (expressed in degrees); d, the
path length (cm); and c, the protein concentration (gL−1).
All CD spectra were corrected for buffer contributions
and secondary structures were calculated by using web
based K2d neural network software package.
where []MRE is the calculated mean residue ellipticity(deg cm2 dmol−1); MRW, the mean residue weight for thepeptide bond [MRW is calculated as MRW = M/N-1,where M is the molecular mass of the polypeptide chain(Da), and N is the number of amino acids in the chain];obs, the observed ellipticity (expressed in degrees); d, thepath length (cm); and c, the protein concentration (gL−1).All CD spectra were corrected for buffer contributionsand secondary structures were calculated by using webbased K2d neural network software package.
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