The object of the present study is to measure the concentrations of toxic heavy metals in human eyes (cadimium and
chromium) and major mineral iron ion. The heavy metals cadimium, chromium and iron were assayed using atomic
absorption spectrometry. Metals determination in human eye is the most common application of biological monitoring
for screening diagnosis and assessment of metals exposures and their risks. The statistical analysis of the metals (cadimium,
chromium and iron) levels in human eyes showed that levels of the three metals in non-smoker were lower than
metals contents of the smoker groups. Metals levels in human eyes of males were significantly lower than females. Statistically
significant differences (p < 0.001) were observed between persons living in city centre and others who living in
outskirt for concentrations of all three metals. Geographical influences are thought to be the main source of variability.