INTRODUCTION
Ginger genera can be grown in their natural habitats and under artificial
conditions, such as greenhouse and in vitro culture. In vitro conservation of ginger genera
is a complementary strategy and safe to protect genetic resources from epidemic diseases
and other natural disasters (Yamuna et al., 2007; Neumann et al., 2009). At the same time,
breeding and plant tissue culture techniques were applied to conserve and increase several
ginger species.
H. coronarium J. König is found in tropical and subtropical regions. It is one of
the important ornamental plants because of the sweet fragrant extract from its white
flowers. In addition, the aromatic oil from H. coronarium rhizomes has antimicrobial
activity (Beena et al., 2007). The conventional propagation of H. coronarium is rare
because the seeds are hardly germinated. Therefore, this species has tendency to be
extinct if conservation and appropriate propagation are not applied.