2.1. Chromatographic Settings
A high performance liquid chromatography device coupled to a fluorescence detector (HPLC-FLD, Agilent Technologies
® 1200 series, Waldbronn, Germany) was used to analyze abamectin, with a Partisil 5 ODS-3 column
(250 × 4.6 mm, 5 μm, Whatman®), at a controlled temperature of 25˚C. The excitation and emission wavelengths
were 364 and 475 nm, respectively. An isocratic mobile phase comprised of ACN:H2O:THF (80:14:6, in volume)
was used, with a flow rate of 1.0 mL·min−1 and a run time of 14 min. The injection volume was 100 μL.