For physical disruption, tubes were placed in a sonicator (ultrasonic bath LBS2; Falc Instruments) at 60 KHz for 3–5 min then heated at 100 uC for 5–10 min.
For physical disruption, tubes wereplaced in a sonicator (ultrasonic bath LBS2; Falc Instruments) at 60KHz for 3–5 min then heated at 100 uC for 5–10 min.