The second is nonspecific lipid transfer proteins Ara h 9 [4].
The Ara h 2, Ara h 7 and Ara h 9 are in the same prolamin superfamily based on the eight cysteine conserved
pattern [5,8], though very low sequence homology. It has been known in the literatures that seed albumins are
extractable in water or diluted salt while globulins are salt soluble extracted by high salt concentration i.e. at
least 1 M NaCl. Prolamins are soluble in aqueous alcohol. The globulin family has been so far identified for
peanut drought tolerant genotypes [9], which are known as the approach to reduce aflatoxin contamination.
Focusing at the Ara h members, the albumin and prolamin families have potential to associate with antifungal
action. It is of our initial intention to extract peanut seed proteins with alternated order of solvent hopefully to
obtain fractions of albumins and prolamins having distinguished profiles. Both fractions were tested against A.
flavus comparing to the globulin fraction. The data will be applied for screening peanut germplasms with an
advantage genotype of antifungal seed storage proteins for the breeding program.