Univariate screenings for a potential application of CIM tube
monolithic columns (BIA separations, Slovenia) as a second chromatographic
purification step were performed with the partially
purified isoenzyme C1A after HCIC. Flowthrough fractions from
HCIC purifications were pooled, concentrated and rebuffered in
either of the loading buffers: 50 mM Tris–HCl, pH 7.4, 50 mM
Tris–HCl, pH 8.0 or 50 mM potassium phosphate, pH 6.0. The
respective elution buffers contained 1 M NaCl.