Briefly, the host for
recombinant DNA manipulations was Escherichia coli
strain NovaBlue (Novagen, Madison, WI, USA), and
cellulolytic enzymes were expressed in the haploid yeast
strains S. cerevisiae MT8-1 [17] and NBRC1440ΔHUWL
[13]. The haploid and diploid S. cerevisiae strains 1440/
cocδBEC3 and MNII/cocδBEC3, respectively, were constructed
as described below.
E. coli transformants were