Compound Chlorhexidine Acetate gel of the present invention discloses a treatment of livestock endometritis and its preparation method, as a percentage of the weight of the gel consists of the following components: chlorhexidine acetate 0.3 to 0.8 percent, metronidazole 0.5 % to 1% Carbomer 0.6% ~ 1.2%, pH adjusting agent: 0.4% to 0.7% of an organic solvent: 30% ~ 50%, solubilizer: from 0.05% to 0.3% preservative: 0.5 to 0.15% the balance being water. The preparation method is in accordance with the above weight percentages of each component ratio, taking carbomer graded sprinkled with water to avoid lumps, stir standing 24 ~ 48h adding a pH adjusting agent to give the matrix. By taking the percentage by weight of chlorhexidine acetate, metronidazole, organic solvents, solubilizers, preservatives, after mixing, is added to the matrix, to obtain purified water to make up the gel. The present invention is administered by the gel adheres to the vaginal mucosa, with the slow diffusion gel drugs prolong duration of action of drugs, the cure rate is high, a significant effect, and ultimately therapeutic purposes.